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Legion Biome Ingredients (1,192 milligrams per serving)

Bifidobacterium Lactis B420® (10 billion CFU per serving)

Bifidobacterium lactis is a strain of probiotic bacteria that reduces the production of toxic compounds that are produced by harmful intestinal bacteria and can cause inflammation throughout the body.[8]

B420® is a patented form of Bifidobacterium lactis that has been extensively studied, with over 60 peer-reviewed studies supporting its efficacy.

Research shows supplementation with B420® Bifidobacterium lactis . . .

  • Decreases intestinal inflammation[9]
  • Reduces food intake and promotes fat loss (particularly in the stomach region) and weight maintenance through mechanisms not fully understood yet, but possibly related to improvements in the function of the gut barrier[10]
  • May improve the function of the gut barrier, which controls the absorption of nutrients, electrolytes, and water, as well as the containment and elimination of toxins and pathogens[11][12][13]

Bifidobacterium lactis B420® also boosts levels of healthful bacteria in the digestive tract as well as concentrations of a protein called GLP-1 that influences the release of insulin from the pancreas (and thus glucose control) and other hormones that impact appetite.[14][15]

The clinically effective dose of Bifidobacterium lactis B420® is 10 billion CFU.

Bacillus Subtilis DE111® (5 billion CFU per serving)

Bacillus subtilis DE111® is a strain of probiotic bacteria that increases the production of white blood cells in response to inflammation, positively influences hormones related to satiety (fullness), and boosts levels of healthy bacteria in the digestive tract.[16][17][18]

DE111® is a patented form of Bacillus subtilis that has been extensively researched, with over 30 peer-reviewed studies supporting its efficacy.

Research shows supplementation with DE111® Bacillus subtilis . . .

  • Increases the body's ability to fight off infections[19]
  • Reduces whole-body inflammation levels[20]
  • Improves cholesterol levels and blood vessel function[21]
  • May promote fat loss through mechanisms not fully understood yet, but possibly related to improvements in the function of the gut barrier[22]

The clinically effective dose of DE111® Bacillus subtilis is 5 billion CFU.

Lactobacillus Plantarum 299v® (10 billion CFU per serving)

Lactobacillus plantarum 299v® is a strain of probiotic bacteria that improves the function of the gut barrier, which controls the absorption of nutrients, electrolytes, and water, as well as the containment and elimination of toxins and pathogens.[23]

Lactobacillus plantarum also reduces the levels of "bad" bacteria in the digestive system and enhances the production of short-chain fatty acids, which nourish intestinal cells.[24]

LP299v® is a patented form of Lactobacillus plantarum that has been more extensively researched than any other form on the market, with over 60 peer-reviewed studies supporting its efficacy.

Research shows supplementation with 299v® Lactobacillus plantarum . . .

  • Reduces abdominal pain, cramping, bloating, and flatulence[25][26]
  • Improves symptoms of Irritable Bowel Syndrome (IBS)[27][28]
  • Improves the body's ability to absorb iron[29]

The clinically effective dose of Lactobacillus plantarum 299v® is 10 billion CFU.

PreticX® Xylooligosaccharide (1,000 milligrams per serving)

A prebiotic is a form of non-digestible fiber that serves as food for beneficial gut bacteria, helping them grow faster and replace the “bad” bacteria in your digestive system.

A xylooligosaccharide is a form of prebiotic that’s naturally found in fruit, vegetables, milk, and honey.

PreticX® is a patented form of xylooligosaccharide that has been extensively researched, with more than a dozen peer-reviewed studies supporting its efficacy. What's more, studies show that PreticX® is more effective at lower doses than most other prebiotics.[30]

Research shows supplementation with PreticX® xylooligosaccharide . . .

  • Increases levels of healthy intestinal bacteria[31]
  • Reduces the growth of gut bacteria that cause inflammation and are associated with an increased risk of type-2 diabetes[32]

The clinically effective dose of PreticX® xylooligosaccharide is 1,000 milligrams.

No Artificial Food Dyes, Fillers, or Other Unnecessary Junk

Artificial food dyes and fillers may not be as dangerous as some people claim, but studies show they can cause negative effects in some people, including gastrointestinal toxicity and behavioral disorders.[33][34][35][36][37]

That’s why we use natural coloring derived from fruits and other foods as well as naturally derived ingredients for enhancing shelf life and facilitating the manufacturing process.

No Artificial Food Dyes or Other Chemical Junk

Third-Party Lab Tested by Labdoor™

Biome is lab-tested for purity and accuracy and independently certified by Labdoor™ to meet or exceed FDA safety guidelines.[38]

Third-Party Lab Tested for Potency & Purity

Made in the USA with Globally Sourced Ingredients

If you want to ensure the supplements you’re swallowing every day are safe and effective, you want to buy from a company that:

  1. Sources ingredients from premium suppliers around the world (great supplements require great raw materials)
  2. Tests all products for purity and accuracy in accredited laboratories (to conclusively verify safety and efficacy)
  3. Manufactures in America, which has some of the strictest regulations in the world

And that’s exactly what we do here at Legion.

Biome Lab Test Certificate
Biome COA

See how Legion Biome compares to the rest.

  • Active Ingredients
  • Clinically Effective Ingredients and Doses
  • Bifidobacterium lactis
  • Bacillus subtilis
  • Lactobacillus plantarum
  • Xylooligosaccharide
  • Third-Party Lab Tested by Labdoor™
  • Labdoor Certified Brand
  • Price Per Serving
  • Legion Biome
    Probiotic

    Legion Biome
  • 1,192 mg
    per serving
  • True
  • 10 billion CFU
    per serving
  • 5 billion CFU
    per serving
  • 10 billion CFU
    per serving
  • 1,000 mg
    per serving
  • True
  • True
  • Transparent Labs
    Probiotic

    Transparent Labs Probiotic
  • 1,000 mg
    per serving
  • False
  • 10 billion CFU
    per serving
  • False
  • 10 billion CFU
    per serving
  • False
  • True
  • True
  • $1.53
  • Garden of Life
    Probiotic

    Garden of Life Probiotic
  • 625 mg
    per serving
  • Question Mark
  • close
    (proprietary blend)

    A proprietary blend is a group of ingredients that lists their names but not amounts, making it impossible to know if the dosages are at clinically effective levels.

  • False
  • close
    (proprietary blend)

    A proprietary blend is a group of ingredients that lists their names but not amounts, making it impossible to know if the dosages are at clinically effective levels.

  • False
  • Question Mark
  • True
  • $1.43
  • NOW Foods
    Probiotic-10

    NOW Foods Probiotic-10
  • 640 mg
    per serving
  • Question Mark
  • close
    (proprietary blend)

    A proprietary blend is a group of ingredients that lists their names but not amounts, making it impossible to know if the dosages are at clinically effective levels.

  • False
  • close
    (proprietary blend)

    A proprietary blend is a group of ingredients that lists their names but not amounts, making it impossible to know if the dosages are at clinically effective levels.

  • False
  • Question Mark
  • False
  • $1.50

The #1 brand of naturally sweetened and flavored sports supplements.

We’ve sold over 5 million bags and bottles to over 1 million customers in 169 countries who have left us over 55,000 5-star reviews.

Science-Backed Ingredients
Clinically Effective Ingredients and Doses

Every active ingredient, form, and dose in Biome is backed by peer-reviewed scientific research demonstrating clear benefits in healthy humans.

Total Label Transparency
Total Label Transparency

We clearly list the dose of each ingredient in Biome on the label—no proprietary blends or hidden ingredients—so you can verify our formulation’s validity and effectiveness.

Lab Tested
Third-Party Lab Tested by Labdoor™

Biome is lab-tested for purity and accuracy and independently certified by Labdoor™ to meet or exceed FDA safety guidelines.

Natural Ingredients
No Unnecessary Junk

Biome contains no artificial food dyes, fillers, or other unnecessary junk.

Made in USA
Made in the USA

Biome is made in America with globally sourced ingredients in NSF-certified, FDA-inspected facilities that adhere to Current Good Manufacturing Practice (cGMP) standards.

100% Money-Back-Guarantee
100% Money-Back Guarantee

If you don't absolutely love Biome, you get a prompt and courteous refund. No forms or returns necessary.

Trusted by scientists, doctors, and everyday fitness folk alike.

  • Dr. Bill Campbell, PhD, FISSN, CSCS

    "I most value Legion's integrity. Their products are based on the ingredients and doses shown to be effective by the scientific studies, and they don't just cite research but also fund it."

    Dr. Bill Campbell, PhD, FISSN, CSCS

    Professor of Exercise Science, University of South Florida
    Chair of Scientific Advisory Board
  • Dr. Jessie Hoffman, PhD, RD, CSDH

    "I recommend Legion because they're transparent about what's in their products, they prioritize evidence-based ingredients and dosing, and they use third-party testing."

    Dr. Jessie Hoffman, PhD, RD, CSDH

    Assistant Professor of Human Nutrition, Winthrop University
    Scientific Advisory Board
  • Dr. Eugene Choi, MD

    "As a physician, I look for evidence, transparency, and consistency. Legion checks all three. I've used their products for over 7 years because their formulations reflect a level of rigor that's uncommon in this space."

    Dr. Eugene Choi, MD

    Chair of Radiology, Good Samaritan Hospital, Los Angeles
    Scientific Advisory Board
  • Dr. Katie Vasenina, PhD, CISSN

    "I trust Legion's approach because every ingredient, dose, and formulation decision is grounded in research on what actually works in real-world application, not just what looks good on a label."

    Dr. Katie Vasenina, PhD, CISSN

    Professor of Exercise Science, University of Tampa
    Lead Formulation Scientist
  • Dr. Alyssa Olenick, PhD, CISSN

    "Legion is one of the few supplement companies with transparency and integrity. You not only get what you pay for, you know what you're paying for and taking and why."

    Dr. Alyssa Olenick, PhD, CISSN

    Exercise, Metabolism, and Female Physiology Scientist
  • Andres Ayesta, MS, RD, LD

    "I've used Legion products for years. It's rare to find a company that combines high-quality supplements with evidence-backed ingredients and fully transparent formulations."

    Andres Ayesta, MS, RD, LD

    Founder of Planos Nutrition
    Scientific Advisory Board
  • Dr. Stacie Barber PT, DPT, CSCS, FRCms, USAW

    "Legion's commitment to transparency in their labeling and use of top-notch ingredients sets them apart from other supplement companies."

    Dr. Stacie Barber PT, DPT, CSCS, FRCms, USAW

    Founder of The Physio Fix
  • Dr. Kasey Jo Orvidas, PhD

    "As someone with a strong academic and research-heavy background, I feel Legion really “gets it.” I know that what I think I'm getting with their products is actually what I'm getting: ingredients and doses supported by evidence. I can buy and recommend anything from Legion without a second thought."

    Dr. Kasey Jo Orvidas, PhD

    Published Researcher and Health Coach
  • Jordan Syatt

    "Legion is a tremendous company run by people I trust and respect. The quality of their supplements is second to no one. And while I don't use many supplements, the only ones I do come from Legion."

    Jordan Syatt

    Strength and Nutrition Coach
  • Menno Henselmans, MS

    "Legion consults with nutrition experts to make honest, science-based products and then proves they're genuine with independent lab work. That's how all supplement companies should operate."

    Menno Henselmans, MS

    Founder of Bayesian Bodybuilding, Published Scientist, and Physique Coach

The actual evidence? Real reviews from verified buyers.

Frequently Asked Questions

How do I use Biome?
What makes Biome different from other probiotics on the market?
Can Biome help me if I don’t have any digestive issues?
What’s the difference between Biome and Balance?
Can I take Biome and Balance together?
How long will it take before I notice results?
Does Biome require refrigeration?
What does the Prop65 warning on the labels mean?
Can I take Biome while pregnant or breastfeeding?
Can Biome be taken with medication?
Is Biome gluten-free? Soy-free? Dairy-free?
Is Biome vegetarian friendly?
Is Biome vegan friendly?

+References

Did you know that some supplements contain dangerously high levels of toxins like lead, arsenic, and cadmium?

That’s why we rigorously test every batch and guarantee our products provide exactly what the labels claim—and nothing else.

With Legion, you can be certain that what you're putting into your body is truly safe and effective.

↑

Science Review: Bifidobacterium lactis 420

Metagenics Institute. Accessed October 16, 2022. ↑

Bifidobacterium animalis subsp. lactis 420 for Metabolic Health: Review of the Research.

Uusitupa HM, Rasinkangas P, Lehtinen MJ, et al. Nutrients. 2020;12(4):892. Published 2020 Mar 25. doi:10.3390/nu12040892.↑

Probiotic With or Without Fiber Controls Body Fat Mass, Associated With Serum Zonulin, in Overweight and Obese Adults-Randomized Controlled Trial.

Stenman LK, Lehtinen MJ, Meland N, et al. EBioMedicine. 2016;13:190-200. doi:10.1016/j.ebiom.2016.10.036.↑

Probiotic or synbiotic alters the gut microbiota and metabolism in a randomised controlled trial of weight management in overweight adults.

Hibberd AA, Yde CC, Ziegler ML, et al. Benef Microbes. 2019;10(2):121-135. doi:10.3920/BM2018.0028.↑

Cross-talk between Akkermansia muciniphila and intestinal epithelium controls diet-induced obesity.

Everard A, Belzer C, Geurts L, et al. Proc Natl Acad Sci U S A. 2013;110(22):9066-9071. doi:10.1073/pnas.1219451110.↑

Probiotic With or Without Fiber Controls Body Fat Mass, Associated With Serum Zonulin, in Overweight and Obese Adults-Randomized Controlled Trial.

Stenman LK, Lehtinen MJ, Meland N, et al. EBioMedicine. 2016;13:190-200. doi:10.1016/j.ebiom.2016.10.036.↑

Science Review: Bifidobacterium lactis 420

Metagenics Institute. Accessed October 16, 2022. ↑

Probiotic or synbiotic alters the gut microbiota and metabolism in a randomised controlled trial of weight management in overweight adults.

Hibberd AA, Yde CC, Ziegler ML, et al. Benef Microbes. 2019;10(2):121-135. doi:10.3920/BM2018.0028.↑

Examining the Gastrointestinal and Immunomodulatory Effects of the Novel Probiotic Bacillus subtilis DE111.

Freedman KE, Hill JL, Wei Y, et al. Int J Mol Sci. 2021;22(5):2453. Published 2021 Feb 28. doi:10.3390/ijms22052453.↑

Effects of Probiotic (Bacillus subtilis) Supplementation During Offseason Resistance Training in Female Division I Athletes.

Toohey JC, Townsend JR, Johnson SB, et al. J Strength Cond Res. 2020;34(11):3173-3181. doi:10.1519/JSC.0000000000002675.↑

Presence and Germination of the Probiotic Bacillus subtilis DE111® in the Human Small Intestinal Tract: A Randomized, Crossover, Double-Blind, and Placebo-Controlled Study.

Colom J, Freitas D, Simon A, et al. Front Microbiol. 2021;12:715863. Published 2021 Aug 2. doi:10.3389/fmicb.2021.715863.↑

Examining the Gastrointestinal and Immunomodulatory Effects of the Novel Probiotic Bacillus subtilis DE111.

Freedman KE, Hill JL, Wei Y, et al. Int J Mol Sci. 2021;22(5):2453. Published 2021 Feb 28. doi:10.3390/ijms22052453.↑

Effects of Probiotic (Bacillus subtilis DE111) Supplementation on Immune Function, Hormonal Status, and Physical Performance in Division I Baseball Players.

Townsend JR, Bender D, Vantrease WC, et al. Sports (Basel). 2018;6(3):70. Published 2018 Jul 26. doi:10.3390/sports6030070.↑

Bacillus subtilis DE111 intake may improve blood lipids and endothelial function in healthy adults.

Trotter RE, Vazquez AR, Grubb DS, et al. Benef Microbes. 2020;11(7):621-630. doi:10.3920/BM2020.0039.↑

Effects of Probiotic (Bacillus subtilis) Supplementation During Offseason Resistance Training in Female Division I Athletes.

Toohey JC, Townsend JR, Johnson SB, et al. J Strength Cond Res. 2020;34(11):3173-3181. doi:10.1519/JSC.0000000000002675.↑

Lactiplantibacillus plantarum 299v (LP299V®): three decades of research.

Nordström EA, Teixeira C, Montelius C, Jeppsson B, Larsson N. Benef Microbes. 2021;12(5):441-465. doi:10.3920/BM2020.0191.↑

Survival of Lactobacillus plantarum DSM 9843 (299v), and effect on the short-chain fatty acid content of faeces after ingestion of a rose-hip drink with fermented oats.

Johansson ML, Nobaek S, Berggren A, et al. Int J Food Microbiol. 1998;42(1-2):29-38. doi:10.1016/S0168-1605(98)00055-5.↑

Clinical trial: Lactobacillus plantarum 299v (DSM 9843) improves symptoms of irritable bowel syndrome.

Ducrotté P, Sawant P, Jayanthi V. World J Gastroenterol. 2012;18(30):4012-4018. doi:10.3748/wjg.v18.i30.4012.↑

Effect of probiotic species on irritable bowel syndrome symptoms: A bring up to date meta-analysis.

Ortiz-Lucas M, Tobías A, Saz P, Sebastián JJ. Rev Esp Enferm Dig. 2013;105(1):19-36. doi:10.4321/s1130-01082013000100005.↑

British Dietetic Association systematic review of systematic reviews and evidence-based practice guidelines for the use of probiotics in the management of irritable bowel syndrome in adults (2016 update).

McKenzie YA, Thompson J, Gulia P, Lomer MC; (IBS Dietetic Guideline Review Group on behalf of Gastroenterology Specialist Group of the British Dietetic Association). J Hum Nutr Diet. 2016;29(5):576-592. doi:10.1111/jhn.12386.↑

Choosing an appropriate probiotic product for your patient: An evidence-based practical guide.

Sniffen JC, McFarland LV, Evans CT, Goldstein EJC. PLoS One. 2018;13(12):e0209205. Published 2018 Dec 26. doi:10.1371/journal.pone.0209205.↑

Xylooligosaccharide increases bifidobacteria but not lactobacilli in human gut microbiota.

Finegold SM, Li Z, Summanen PH, et al. Food Funct. 2014;5(3):436-445. doi:10.1039/c3fo60348b.↑

Xylooligosaccharide increases bifidobacteria but not lactobacilli in human gut microbiota.

Finegold SM, Li Z, Summanen PH, et al. Food Funct. 2014;5(3):436-445. doi:10.1039/c3fo60348b.↑

Xylooligosaccharide supplementation alters gut bacteria in both healthy and prediabetic adults: a pilot study.

Yang J, Summanen PH, Henning SM, et al. Front Physiol. 2015;6:216. Published 2015 Aug 7. doi:10.3389/fphys.2015.00216.↑

Toxicological significance of azo dye metabolism by human intestinal microbiota.

Feng J, Cerniglia CE, Chen H. Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, AR , USA. Front Biosci (Elite Ed). 2012 Jan 1;4:568-86. ↑

Artificial food dyes and attention deficit hyperactivity disorder.

Kanarek RB. Department of Psychology, Tufts University, Medford, Massachusetts, USA. Nutr Rev. 2011 Jul;69(7):385-91. ↑

Meta-analysis of attention-deficit/hyperactivity disorder or attention-deficit/hyperactivity disorder symptoms, restriction diet, and synthetic food color additives.

Nigg JT, Lewis K, Edinger T, Falk M. Oregon Health and Science University, Portland, OR, USA. J Am Acad Child Adolesc Psychiatry. 2012 Jan;51(1):86-97.e8. ↑

Food additives and hyperactive behaviour in 3-year-old and 8/9-year-old children in the community: a randomised, double-blinded, placebo-controlled trial.

McCann D, Barrett A, Cooper A, Crumpler D, Dalen L, Grimshaw K, Kitchin E, Lok K, Porteous L, Prince E, Sonuga-Barke E, Warner JO, Stevenson J. School of Psychology, Department of Child Health, University of Southampton, Southampton, UK. Lancet. 2007 Nov 3;370(9598):1560-7. ↑

Effect of food azo dye tartrazine on learning and memory functions in mice and rats, and the possible mechanisms involved.

Gao Y, Li C, Shen J, Yin H, An X, Jin H. Scientific and Technological College of Chemistry and Biology, Yantai Univ., Yantai, PR China. J Food Sci. 2011 Aug;76(6):T125-9. ↑